The objective of the present research work was to optimize the preparation of ritonavir loaded albumin nanoparticles by modified desolvation technique using response surface methodology. A box-behnken design with 3 factors and 3 levels were used in the study. The albumin concentration, pH and rate of ethanol were selected as independent variables, loading capacity; entrapment efficiency and release of drug were chosen as response variables. The optimized formulation of RV was composed of 9.5% BSA, pH at 7.4 and 1.4 ml/ min of ethanol rate. The optimized formulation showed mean particle size of 163.1 nm, zeta potential of -23.9 mV, loading efficiency 31.23%, entrapment efficiency 76.80% and release of drug at 12 h 58.23%. In-vitro experiments showed a burst release at the initial stage and followed by prolonged release of RV from the nanoparticles up to 24 h.
Loading....